S-BIAD855alpha

Human U2OS cells - compound-profiling Cell Painting experiment (OME-NGFF)

Released 2023-08-22
Image Data Resource (IDR)

Content

  • 20 images
  • Study size: 169.1GiB
  • N files (exc zip contents): 20
  • N files (inc zip contents): 20
  • Filetype breakdown:
    • .ome.zarr.zip: 20 (169.1GiB)
OME-NGFF converted study from idr0036. Computational methods for image-based profiling are under active development, but their success hinges on assays that can capture a wide range of phenotypes. We have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery. Note: This image set is BBBC022v1 [Gustafsdottir et al., PLOS ONE, 2013], obtained from the Broad Bioimage Benchmark Collection [Ljosa et al., Nature Methods, 2012].

Study information

Organism
Homo sapiens (human)
Study type
Imaging type
fluorescence microscopy

Viewable images

Image ID Preview Filename Dimensions
(T, C, Z, Y, X)		 Download Size Actions
IM1 idr0036/20596.ome.zarr.zip None 8.7GiB
IM2 idr0036/20591.ome.zarr.zip None 8.7GiB
IM3 idr0036/20633.ome.zarr.zip None 8.2GiB
IM4 idr0036/20589.ome.zarr.zip None 8.7GiB
IM5 idr0036/20641.ome.zarr.zip None 8.2GiB
IM6 idr0036/20593.ome.zarr.zip None 8.5GiB
IM7 idr0036/20608.ome.zarr.zip None 8.3GiB
IM8 idr0036/20595.ome.zarr.zip None 8.6GiB
IM9 idr0036/20585.ome.zarr.zip None 8.6GiB
IM10 idr0036/20639.ome.zarr.zip None 8.4GiB
IM11 idr0036/20625.ome.zarr.zip None 8.5GiB
IM12 idr0036/20607.ome.zarr.zip None 8.5GiB
IM13 idr0036/20594.ome.zarr.zip None 8.7GiB
IM14 idr0036/20646.ome.zarr.zip None 8.1GiB
IM15 idr0036/20590.ome.zarr.zip None 8.5GiB
IM16 idr0036/20640.ome.zarr.zip None 8.3GiB
IM17 idr0036/20626.ome.zarr.zip None 8.3GiB
IM18 idr0036/20592.ome.zarr.zip None 8.6GiB
IM19 idr0036/20630.ome.zarr.zip None 8.5GiB
IM20 idr0036/20586.ome.zarr.zip None 8.3GiB

All images

Image ID Filename Download Size Actions
IM1 idr0036/20596.ome.zarr.zip 8.7GiB Download
IM2 idr0036/20591.ome.zarr.zip 8.7GiB Download
IM3 idr0036/20633.ome.zarr.zip 8.2GiB Download
IM4 idr0036/20589.ome.zarr.zip 8.7GiB Download
IM5 idr0036/20641.ome.zarr.zip 8.2GiB Download
IM6 idr0036/20593.ome.zarr.zip 8.5GiB Download
IM7 idr0036/20608.ome.zarr.zip 8.3GiB Download
IM8 idr0036/20595.ome.zarr.zip 8.6GiB Download
IM9 idr0036/20585.ome.zarr.zip 8.6GiB Download
IM10 idr0036/20639.ome.zarr.zip 8.4GiB Download
IM11 idr0036/20625.ome.zarr.zip 8.5GiB Download
IM12 idr0036/20607.ome.zarr.zip 8.5GiB Download
IM13 idr0036/20594.ome.zarr.zip 8.7GiB Download
IM14 idr0036/20646.ome.zarr.zip 8.1GiB Download
IM15 idr0036/20590.ome.zarr.zip 8.5GiB Download
IM16 idr0036/20640.ome.zarr.zip 8.3GiB Download
IM17 idr0036/20626.ome.zarr.zip 8.3GiB Download
IM18 idr0036/20592.ome.zarr.zip 8.6GiB Download
IM19 idr0036/20630.ome.zarr.zip 8.5GiB Download
IM20 idr0036/20586.ome.zarr.zip 8.3GiB Download