S-BIAD847alpha

High-Content Phenotypic Profiling of Drug Response Signatures across Distinct Cancer Cells (OME-NGFF)

Released 2023-08-14
Image Data Resource (IDR)

Content

  • 55 images
  • Study size: 68.9GiB
  • N files (exc zip contents): 55
  • N files (inc zip contents): 55
  • Filetype breakdown:
    • .zarr.zip: 55 (68.9GiB)
OME-NGFF converted study from idr0035. The application of high-content imaging in conjunction with multivariate clustering techniques has recently shown value in the confirmation of cellular activity and further characterization of drug mode of action following pharmacologic perturbation. However, such practical examples of phenotypic profiling of drug response published to date have largely been restricted to cell lines and phenotypic response markers that are amenable to basic cellular imaging. As such, these approaches preclude the analysis of both complex heterogeneous phenotypic responses and subtle changes in cell morphology across physiologically relevant cell panels. Here, we describe the application of a cell-based assay and custom designed image analysis algorithms designed to monitor morphologic phenotypic response in detail across distinct cancer cell types. We further describe the integration of these methods with automated data analysis workflows incorporating principal component analysis, Kohonen neural networking, and kNN classification to enable rapid and robust interrogation of such data sets. We show the utility of these approaches by providing novel insight into pharmacologic response across four cancer cell types, Ovcar3, MiaPaCa2, and MCF7 cells wild-type and mutant for p53. These methods have the potential to drive the development of a new generation of novel therapeutic classes encompassing pharmacologic compositions or polypharmacology in appropriate disease context. Note: The images in idr0035/screenA were generated by Caie et al, 2010 and are from MCF7 wild-type cells only. They were obtained from the Broad BioImage Benchmark Collection image set BBBC021v1. (https://data.broadinstitute.org/bbbc/BBBC021/) submitted by Ljosa et al, 2013. The Mechanism of Action ground truth data comes from Ljosa et al, 2013.

Study information

Organism
Homo sapiens (human)
Study type
Imaging type
fluorescence microscopy

Viewable images

Image ID Preview Filename Dimensions
(T, C, Z, Y, X)		 Download Size Actions
IM1 idr0035/Week3_25421.zarr.zip None 1.3GiB
IM2 idr0035/Week9_39282.zarr.zip None 1.1GiB
IM3 idr0035/Week9_39221.zarr.zip None 1.2GiB
IM4 idr0035/Week1_22361.zarr.zip None 1.3GiB
IM5 idr0035/Week7_34681.zarr.zip None 1.3GiB
IM6 idr0035/Week10_40115.zarr.zip None 1.3GiB
IM7 idr0035/Week4_27481.zarr.zip None 1.2GiB
IM8 idr0035/Week3_25701.zarr.zip None 1.3GiB
IM9 idr0035/Week7_34641.zarr.zip None 1.3GiB
IM10 idr0035/Week5_28921.zarr.zip None 1.3GiB
IM11 idr0035/Week3_25461.zarr.zip None 1.3GiB
IM12 idr0035/Week8_38341.zarr.zip None 1.2GiB
IM13 idr0035/Week7_34661.zarr.zip None 1.3GiB
IM14 idr0035/Week1_22161.zarr.zip None 1.3GiB
IM15 idr0035/Week6_31641.zarr.zip None 1.2GiB
IM16 idr0035/Week6_32161.zarr.zip None 1.2GiB
IM17 idr0035/Week8_38241.zarr.zip None 1.2GiB
IM18 idr0035/Week4_27542.zarr.zip None 1.3GiB
IM19 idr0035/Week8_38203.zarr.zip None 1.2GiB
IM20 idr0035/Week2_24401.zarr.zip None 1.2GiB
IM21 idr0035/Week9_39222.zarr.zip None 1.2GiB
IM22 idr0035/Week1_22381.zarr.zip None 1.3GiB
IM23 idr0035/Week6_32061.zarr.zip None 1.2GiB
IM24 idr0035/Week7_34341.zarr.zip None 1.3GiB
IM25 idr0035/Week5_28901.zarr.zip None 1.3GiB
IM26 idr0035/Week4_27821.zarr.zip None 1.3GiB
IM27 idr0035/Week10_40119.zarr.zip None 1.3GiB
IM28 idr0035/Week5_29301.zarr.zip None 1.3GiB
IM29 idr0035/Week3_25441.zarr.zip None 1.3GiB
IM30 idr0035/Week7_34381.zarr.zip None 1.2GiB
IM31 idr0035/Week9_39206.zarr.zip None 1.2GiB
IM32 idr0035/Week4_27521.zarr.zip None 1.3GiB
IM33 idr0035/Week10_40111.zarr.zip None 1.3GiB
IM34 idr0035/Week8_38221.zarr.zip None 1.3GiB
IM35 idr0035/Week3_25681.zarr.zip None 1.3GiB
IM36 idr0035/Week2_24161.zarr.zip None 1.2GiB
IM37 idr0035/Week6_32121.zarr.zip None 1.2GiB
IM38 idr0035/Week9_39283.zarr.zip None 1.2GiB
IM39 idr0035/Week4_27801.zarr.zip None 1.3GiB
IM40 idr0035/Week2_24381.zarr.zip None 1.2GiB
IM41 idr0035/Week4_27861.zarr.zip None 1.3GiB
IM42 idr0035/Week2_24141.zarr.zip None 1.2GiB
IM43 idr0035/Week6_31681.zarr.zip None 1.2GiB
IM44 idr0035/Week2_24121.zarr.zip None 1.2GiB
IM45 idr0035/Week3_25721.zarr.zip None 1.3GiB
IM46 idr0035/Week1_22123.zarr.zip None 1.3GiB
IM47 idr0035/Week8_38342.zarr.zip None 1.2GiB
IM48 idr0035/Week6_31661.zarr.zip None 1.3GiB
IM49 idr0035/Week1_22141.zarr.zip None 1.3GiB
IM50 idr0035/Week9_39301.zarr.zip None 1.1GiB
IM51 idr0035/Week2_24361.zarr.zip None 1.2GiB
IM52 idr0035/Week5_29341.zarr.zip None 1.3GiB
IM53 idr0035/Week5_29321.zarr.zip None 1.3GiB
IM54 idr0035/Week5_28961.zarr.zip None 1.3GiB
IM55 idr0035/Week1_22401.zarr.zip None 1.3GiB

All images

Image ID Filename Download Size Actions
IM1 idr0035/Week3_25421.zarr.zip 1.3GiB Download
IM2 idr0035/Week9_39282.zarr.zip 1.1GiB Download
IM3 idr0035/Week9_39221.zarr.zip 1.2GiB Download
IM4 idr0035/Week1_22361.zarr.zip 1.3GiB Download
IM5 idr0035/Week7_34681.zarr.zip 1.3GiB Download
IM6 idr0035/Week10_40115.zarr.zip 1.3GiB Download
IM7 idr0035/Week4_27481.zarr.zip 1.2GiB Download
IM8 idr0035/Week3_25701.zarr.zip 1.3GiB Download
IM9 idr0035/Week7_34641.zarr.zip 1.3GiB Download
IM10 idr0035/Week5_28921.zarr.zip 1.3GiB Download
IM11 idr0035/Week3_25461.zarr.zip 1.3GiB Download
IM12 idr0035/Week8_38341.zarr.zip 1.2GiB Download
IM13 idr0035/Week7_34661.zarr.zip 1.3GiB Download
IM14 idr0035/Week1_22161.zarr.zip 1.3GiB Download
IM15 idr0035/Week6_31641.zarr.zip 1.2GiB Download
IM16 idr0035/Week6_32161.zarr.zip 1.2GiB Download
IM17 idr0035/Week8_38241.zarr.zip 1.2GiB Download
IM18 idr0035/Week4_27542.zarr.zip 1.3GiB Download
IM19 idr0035/Week8_38203.zarr.zip 1.2GiB Download
IM20 idr0035/Week2_24401.zarr.zip 1.2GiB Download
IM21 idr0035/Week9_39222.zarr.zip 1.2GiB Download
IM22 idr0035/Week1_22381.zarr.zip 1.3GiB Download
IM23 idr0035/Week6_32061.zarr.zip 1.2GiB Download
IM24 idr0035/Week7_34341.zarr.zip 1.3GiB Download
IM25 idr0035/Week5_28901.zarr.zip 1.3GiB Download
IM26 idr0035/Week4_27821.zarr.zip 1.3GiB Download
IM27 idr0035/Week10_40119.zarr.zip 1.3GiB Download
IM28 idr0035/Week5_29301.zarr.zip 1.3GiB Download
IM29 idr0035/Week3_25441.zarr.zip 1.3GiB Download
IM30 idr0035/Week7_34381.zarr.zip 1.2GiB Download
IM31 idr0035/Week9_39206.zarr.zip 1.2GiB Download
IM32 idr0035/Week4_27521.zarr.zip 1.3GiB Download
IM33 idr0035/Week10_40111.zarr.zip 1.3GiB Download
IM34 idr0035/Week8_38221.zarr.zip 1.3GiB Download
IM35 idr0035/Week3_25681.zarr.zip 1.3GiB Download
IM36 idr0035/Week2_24161.zarr.zip 1.2GiB Download
IM37 idr0035/Week6_32121.zarr.zip 1.2GiB Download
IM38 idr0035/Week9_39283.zarr.zip 1.2GiB Download
IM39 idr0035/Week4_27801.zarr.zip 1.3GiB Download
IM40 idr0035/Week2_24381.zarr.zip 1.2GiB Download
IM41 idr0035/Week4_27861.zarr.zip 1.3GiB Download
IM42 idr0035/Week2_24141.zarr.zip 1.2GiB Download
IM43 idr0035/Week6_31681.zarr.zip 1.2GiB Download
IM44 idr0035/Week2_24121.zarr.zip 1.2GiB Download
IM45 idr0035/Week3_25721.zarr.zip 1.3GiB Download
IM46 idr0035/Week1_22123.zarr.zip 1.3GiB Download
IM47 idr0035/Week8_38342.zarr.zip 1.2GiB Download
IM48 idr0035/Week6_31661.zarr.zip 1.3GiB Download
IM49 idr0035/Week1_22141.zarr.zip 1.3GiB Download
IM50 idr0035/Week9_39301.zarr.zip 1.1GiB Download
IM51 idr0035/Week2_24361.zarr.zip 1.2GiB Download
IM52 idr0035/Week5_29341.zarr.zip 1.3GiB Download
IM53 idr0035/Week5_29321.zarr.zip 1.3GiB Download
IM54 idr0035/Week5_28961.zarr.zip 1.3GiB Download
IM55 idr0035/Week1_22401.zarr.zip 1.3GiB Download