S-BIAD845alpha

Clustering phenotype populations by genome-wide RNAi and multiparametric imaging (OME-NGFF)

Released 2023-08-15
Image Data Resource (IDR)

Content

  • 68 images
  • Study size: 317.6GiB
  • N files (exc zip contents): 68
  • N files (inc zip contents): 68
  • Filetype breakdown:
    • .ome.zarr.zip: 68 (317.6GiB)
OME-NGFF converted study from idr0012. Genetic screens for phenotypic similarity have made key contributions to associating genes with biological processes. With RNA interference (RNAi), highly parallel phenotyping of loss-of-function effects in cells has become feasible. One of the current challenges however is the computational categorization of visual phenotypes and the prediction of biological function and processes. In this study, we describe a combined computational and experimental approach to discover novel gene functions and explore functional relationships. We performed a genome-wide RNAi screen in human cells and used quantitative descriptors derived from high-throughput imaging to generate multiparametric phenotypic profiles. We show that profiles predicted functions of genes by phenotypic similarity. Specifically, we examined several candidates including the largely uncharacterized gene DONSON, which shared phenotype similarity with known factors of DNA damage response (DDR) and genomic integrity. Experimental evidence supports that DONSON is a novel centrosomal protein required for DDR signalling and genomic integrity. Multiparametric phenotyping by automated imaging and computational annotation is a powerful method for functional discovery and mapping the landscape of phenotypic responses to cellular perturbations.

Study information

Organism
Homo sapiens (human)
Study type
Imaging type
fluorescence microscopy

Viewable images

Image ID Preview Filename Dimensions
(T, C, Z, Y, X)		 Download Size Actions
IM1 idr0012/HT22.ome.zarr.zip None 4.7GiB
IM2 idr0012/HT14.ome.zarr.zip None 4.6GiB
IM3 idr0012/HT57.ome.zarr.zip None 4.8GiB
IM4 idr0012/HT54.ome.zarr.zip None 4.6GiB
IM5 idr0012/HT23.ome.zarr.zip None 4.6GiB
IM6 idr0012/HT66.ome.zarr.zip None 4.6GiB
IM7 idr0012/HT37.ome.zarr.zip None 4.9GiB
IM8 idr0012/HT63.ome.zarr.zip None 4.6GiB
IM9 idr0012/HT03.ome.zarr.zip None 4.7GiB
IM10 idr0012/HT55.ome.zarr.zip None 4.6GiB
IM11 idr0012/HT21.ome.zarr.zip None 4.7GiB
IM12 idr0012/HT45.ome.zarr.zip None 4.6GiB
IM13 idr0012/HT25.ome.zarr.zip None 4.5GiB
IM14 idr0012/HT07.ome.zarr.zip None 4.7GiB
IM15 idr0012/HT68.ome.zarr.zip None 4.6GiB
IM16 idr0012/HT05.ome.zarr.zip None 4.6GiB
IM17 idr0012/HT10.ome.zarr.zip None 4.6GiB
IM18 idr0012/HT43.ome.zarr.zip None 4.6GiB
IM19 idr0012/HT35.ome.zarr.zip None 4.7GiB
IM20 idr0012/HT01.ome.zarr.zip None 4.8GiB
IM21 idr0012/HT12.ome.zarr.zip None 4.7GiB
IM22 idr0012/HT41.ome.zarr.zip None 4.7GiB
IM23 idr0012/HT31.ome.zarr.zip None 4.8GiB
IM24 idr0012/HT36.ome.zarr.zip None 4.9GiB
IM25 idr0012/HT11.ome.zarr.zip None 4.7GiB
IM26 idr0012/HT18.ome.zarr.zip None 4.7GiB
IM27 idr0012/HT62.ome.zarr.zip None 4.7GiB
IM28 idr0012/HT20.ome.zarr.zip None 4.5GiB
IM29 idr0012/HT39.ome.zarr.zip None 4.8GiB
IM30 idr0012/HT15.ome.zarr.zip None 4.6GiB
IM31 idr0012/HT65.ome.zarr.zip None 4.7GiB
IM32 idr0012/HT48.ome.zarr.zip None 4.7GiB
IM33 idr0012/HT08.ome.zarr.zip None 4.6GiB
IM34 idr0012/HT58.ome.zarr.zip None 4.7GiB
IM35 idr0012/HT34.ome.zarr.zip None 4.6GiB
IM36 idr0012/HT19.ome.zarr.zip None 4.6GiB
IM37 idr0012/HT51.ome.zarr.zip None 4.7GiB
IM38 idr0012/HT04.ome.zarr.zip None 4.7GiB
IM39 idr0012/HT33.ome.zarr.zip None 4.7GiB
IM40 idr0012/HT26.ome.zarr.zip None 4.7GiB
IM41 idr0012/HT13.ome.zarr.zip None 4.7GiB
IM42 idr0012/HT28.ome.zarr.zip None 4.7GiB
IM43 idr0012/HT32.ome.zarr.zip None 4.8GiB
IM44 idr0012/HT27.ome.zarr.zip None 4.7GiB
IM45 idr0012/HT17.ome.zarr.zip None 4.7GiB
IM46 idr0012/HT47.ome.zarr.zip None 4.6GiB
IM47 idr0012/HT44.ome.zarr.zip None 4.6GiB
IM48 idr0012/HT53.ome.zarr.zip None 4.7GiB
IM49 idr0012/HT46.ome.zarr.zip None 4.7GiB
IM50 idr0012/HT38.ome.zarr.zip None 4.9GiB
IM51 idr0012/HT06.ome.zarr.zip None 4.7GiB
IM52 idr0012/HT49.ome.zarr.zip None 4.6GiB
IM53 idr0012/HT67.ome.zarr.zip None 4.6GiB
IM54 idr0012/HT24.ome.zarr.zip None 4.6GiB
IM55 idr0012/HT02.ome.zarr.zip None 4.7GiB
IM56 idr0012/HT52.ome.zarr.zip None 4.7GiB
IM57 idr0012/HT59.ome.zarr.zip None 4.7GiB
IM58 idr0012/HT29.ome.zarr.zip None 4.6GiB
IM59 idr0012/HT64.ome.zarr.zip None 4.6GiB
IM60 idr0012/HT40.ome.zarr.zip None 4.8GiB
IM61 idr0012/HT60.ome.zarr.zip None 4.7GiB
IM62 idr0012/HT56.ome.zarr.zip None 4.8GiB
IM63 idr0012/HT61.ome.zarr.zip None 4.7GiB
IM64 idr0012/HT42.ome.zarr.zip None 4.7GiB
IM65 idr0012/HT16.ome.zarr.zip None 4.7GiB
IM66 idr0012/HT50.ome.zarr.zip None 4.6GiB
IM67 idr0012/HT09.ome.zarr.zip None 4.6GiB
IM68 idr0012/HT30.ome.zarr.zip None 4.5GiB

All images

Image ID Filename Download Size Actions
IM1 idr0012/HT22.ome.zarr.zip 4.7GiB Download
IM2 idr0012/HT14.ome.zarr.zip 4.6GiB Download
IM3 idr0012/HT57.ome.zarr.zip 4.8GiB Download
IM4 idr0012/HT54.ome.zarr.zip 4.6GiB Download
IM5 idr0012/HT23.ome.zarr.zip 4.6GiB Download
IM6 idr0012/HT66.ome.zarr.zip 4.6GiB Download
IM7 idr0012/HT37.ome.zarr.zip 4.9GiB Download
IM8 idr0012/HT63.ome.zarr.zip 4.6GiB Download
IM9 idr0012/HT03.ome.zarr.zip 4.7GiB Download
IM10 idr0012/HT55.ome.zarr.zip 4.6GiB Download
IM11 idr0012/HT21.ome.zarr.zip 4.7GiB Download
IM12 idr0012/HT45.ome.zarr.zip 4.6GiB Download
IM13 idr0012/HT25.ome.zarr.zip 4.5GiB Download
IM14 idr0012/HT07.ome.zarr.zip 4.7GiB Download
IM15 idr0012/HT68.ome.zarr.zip 4.6GiB Download
IM16 idr0012/HT05.ome.zarr.zip 4.6GiB Download
IM17 idr0012/HT10.ome.zarr.zip 4.6GiB Download
IM18 idr0012/HT43.ome.zarr.zip 4.6GiB Download
IM19 idr0012/HT35.ome.zarr.zip 4.7GiB Download
IM20 idr0012/HT01.ome.zarr.zip 4.8GiB Download
IM21 idr0012/HT12.ome.zarr.zip 4.7GiB Download
IM22 idr0012/HT41.ome.zarr.zip 4.7GiB Download
IM23 idr0012/HT31.ome.zarr.zip 4.8GiB Download
IM24 idr0012/HT36.ome.zarr.zip 4.9GiB Download
IM25 idr0012/HT11.ome.zarr.zip 4.7GiB Download
IM26 idr0012/HT18.ome.zarr.zip 4.7GiB Download
IM27 idr0012/HT62.ome.zarr.zip 4.7GiB Download
IM28 idr0012/HT20.ome.zarr.zip 4.5GiB Download
IM29 idr0012/HT39.ome.zarr.zip 4.8GiB Download
IM30 idr0012/HT15.ome.zarr.zip 4.6GiB Download
IM31 idr0012/HT65.ome.zarr.zip 4.7GiB Download
IM32 idr0012/HT48.ome.zarr.zip 4.7GiB Download
IM33 idr0012/HT08.ome.zarr.zip 4.6GiB Download
IM34 idr0012/HT58.ome.zarr.zip 4.7GiB Download
IM35 idr0012/HT34.ome.zarr.zip 4.6GiB Download
IM36 idr0012/HT19.ome.zarr.zip 4.6GiB Download
IM37 idr0012/HT51.ome.zarr.zip 4.7GiB Download
IM38 idr0012/HT04.ome.zarr.zip 4.7GiB Download
IM39 idr0012/HT33.ome.zarr.zip 4.7GiB Download
IM40 idr0012/HT26.ome.zarr.zip 4.7GiB Download
IM41 idr0012/HT13.ome.zarr.zip 4.7GiB Download
IM42 idr0012/HT28.ome.zarr.zip 4.7GiB Download
IM43 idr0012/HT32.ome.zarr.zip 4.8GiB Download
IM44 idr0012/HT27.ome.zarr.zip 4.7GiB Download
IM45 idr0012/HT17.ome.zarr.zip 4.7GiB Download
IM46 idr0012/HT47.ome.zarr.zip 4.6GiB Download
IM47 idr0012/HT44.ome.zarr.zip 4.6GiB Download
IM48 idr0012/HT53.ome.zarr.zip 4.7GiB Download
IM49 idr0012/HT46.ome.zarr.zip 4.7GiB Download
IM50 idr0012/HT38.ome.zarr.zip 4.9GiB Download
IM51 idr0012/HT06.ome.zarr.zip 4.7GiB Download
IM52 idr0012/HT49.ome.zarr.zip 4.6GiB Download
IM53 idr0012/HT67.ome.zarr.zip 4.6GiB Download
IM54 idr0012/HT24.ome.zarr.zip 4.6GiB Download
IM55 idr0012/HT02.ome.zarr.zip 4.7GiB Download
IM56 idr0012/HT52.ome.zarr.zip 4.7GiB Download
IM57 idr0012/HT59.ome.zarr.zip 4.7GiB Download
IM58 idr0012/HT29.ome.zarr.zip 4.6GiB Download
IM59 idr0012/HT64.ome.zarr.zip 4.6GiB Download
IM60 idr0012/HT40.ome.zarr.zip 4.8GiB Download
IM61 idr0012/HT60.ome.zarr.zip 4.7GiB Download
IM62 idr0012/HT56.ome.zarr.zip 4.8GiB Download
IM63 idr0012/HT61.ome.zarr.zip 4.7GiB Download
IM64 idr0012/HT42.ome.zarr.zip 4.7GiB Download
IM65 idr0012/HT16.ome.zarr.zip 4.7GiB Download
IM66 idr0012/HT50.ome.zarr.zip 4.6GiB Download
IM67 idr0012/HT09.ome.zarr.zip 4.6GiB Download
IM68 idr0012/HT30.ome.zarr.zip 4.5GiB Download