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B lymphocytes recognize bacterial or viral antigens via different classes of the B cell antigen receptor (BCR). Protrusive structures termed microvilli cover lymphocyte surfaces and are thought to perform sensory functions in screening antigen-bearing surfaces. Here, we have studied the cell surface topography of Ramos B cells and the spatiotemporal organization of the IgM-BCR using lattice light sheet microscopy in combination with tailored custom-built 4D image analysis. Ramos B cell surfaces were found to form dynamic networks of elevated ridges bridging individual microvilli. A proportion of membrane-localized IgM-BCR was found in clusters, which were mainly associated with the ridges and the microvilli. The dynamic ridge network organization and the IgM-BCR cluster mobility were linked and both were controlled by Arp2/3 complex activity. Our results suggest that dynamic topographical features of the cell surface govern the localization and transport of IgM-BCR clusters to facilitate antigen screening.

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Image ID	Preview	Filename	Dimensions (T, C, Z, Y, X)	Download Size	Actions
IM1		Data/UT/SNAP_IgM_BCR_Cell_1.tif	(35, 2, 100, 320, 525)	Unavailable	view download
IM11		Data/CK666/SNAP-IgM-BCR_Cell_11.tif	(30, 2, 100, 320, 540)	Unavailable	view download
IM13		Data/CK666/SNAP-IgM-BCR_Cell_13.tif	(30, 2, 100, 320, 192)	Unavailable	view download
IM19		Data/NIP/SNAP-IgM-BCR_Cell_19.tif	(50, 2, 75, 284, 258)	Unavailable	view download

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IM1	Data/UT/SNAP_IgM_BCR_Cell_1.tif	Unavailable	Download
IM2	Data/UT/SNAP_IgM_BCR_Cell_2.tif	Unavailable	Download
IM3	Data/UT/SNAP_IgM_BCR_Cell_3.tif	Unavailable	Download
IM4	Data/UT/SNAP_IgM_BCR_Cell_4.tif	Unavailable	Download
IM5	Data/UT/SNAP_IgM_BCR_Cell_5.tif	Unavailable	Download
IM6	Data/UT/SNAP_IgM_BCR_Cell_6.tif	Unavailable	Download
IM7	Data/UT/SNAP_IgM_BCR_Cell_7.tif	Unavailable	Download
IM8	Data/UT/SNAP_CD40_Cell_8.tif	Unavailable	Download
IM9	Data/UT/SNAP_CD40_Cell_9.tif	Unavailable	Download
IM10	Data/UT/SNAP_CD40_Cell_10.tif	Unavailable	Download
IM11	Data/CK666/SNAP-IgM-BCR_Cell_11.tif	Unavailable	Download
IM12	Data/CK666/SNAP-IgM-BCR_Cell_12.tif	Unavailable	Download
IM13	Data/CK666/SNAP-IgM-BCR_Cell_13.tif	Unavailable	Download
IM14	Data/CK666/SNAP-IgM-BCR_Cell_14.tif	Unavailable	Download
IM15	Data/CK666/SNAP-IgM-BCR_Cell_15.tif	Unavailable	Download
IM16	Data/NIP/SNAP-IgM-BCR_Cell_16.tif	Unavailable	Download
IM17	Data/NIP/SNAP-IgM-BCR_Cell_17.tif	Unavailable	Download
IM18	Data/NIP/SNAP-IgM-BCR_Cell_18.tif	Unavailable	Download
IM19	Data/NIP/SNAP-IgM-BCR_Cell_19.tif	Unavailable	Download
IM20	Data/NIP/SNAP-IgM-BCR_Cell_20.tif	Unavailable	Download
IM21	Data/NIP/SNAP-IgM-BCR_Cell_21.tif	Unavailable	Download
IM22	Data/NIP/SNAP-IgM-BCR_Cell_22.tif	Unavailable	Download
IM23	Data/NIP/SNAP-IgM-BCR_Cell_23.tif	Unavailable	Download
IM24	Data/LatA/SNAP_IgM_BCR_Cell_24.tif	Unavailable	Download
IM25	Data/LatA/SNAP_IgM_BCR_Cell_25.tif	Unavailable	Download
IM26	Data/LatA/SNAP_IgM_BCR_Cell_26.tif	Unavailable	Download
IM27	Data/LatA/SNAP_IgM_BCR_Cell_27.tif	Unavailable	Download
IM28	Data/LatA/SNAP_IgM_BCR_Cell_28.tif	Unavailable	Download
IM29	Data/LatA/SNAP_IgM_BCR_Cell_29.tif	Unavailable	Download
IM30	Data/LatA_NIP/SNAP-IgM-BCR_Cell_30.tif	Unavailable	Download
IM31	Data/LatA_NIP/SNAP-IgM-BCR_Cell_31.tif	Unavailable	Download
IM32	Data/LatA_NIP/SNAP-IgM-BCR_Cell_32.tif	Unavailable	Download
IM33	Data/LatA_NIP/SNAP-IgM-BCR_Cell_33.tif	Unavailable	Download
IM34	Data/LatA_NIP/SNAP-IgM-BCR_Cell_34.tif	Unavailable	Download
IM35	Data/LatA_NIP/SNAP-IgM-BCR_Cell_35.tif	Unavailable	Download
IM36	Data/LatA_NIP/SNAP-IgM-BCR_Cell_36.tif	Unavailable	Download
IM37	Data/Noc/SNAP_IgM_BCR_Cell37.tif	Unavailable	Download
IM38	Data/Noc_LatA/SNAP_IgM_BCR_Cell38.tif	Unavailable	Download
IM39	Data/LatA_Noc/SNAP_IgM_BCR_Cell39.tif	Unavailable	Download
IM40	Data/Noc_LatA_NIP/SNAP_IgM_BCR_Cell40.tif	Unavailable	Download
IM41	Data/LatA_Noc_NIP/SNAP_IgM_BCR_Cell41.tif	Unavailable	Download